Anggun Permata Sari, Rara Putri Maliza, Desi Purnama Sari, Anggi Yusriana, Tika Gustina, Abdirrohim Muhammad Qori, Bekir Car, Ratko Pavlović, Jose Vicente Garcia Jimenez, Randi Kurniawan
Background. Aerobic exercise in an enclosed space requires much oxygen for energy metabolism. If oxygen needs are not met, the production of free radicals will increase, causing oxidative stress and can trigger an increase in leukocyte levels. Objectives. This study aims to determine the effect of oxygen administration on closed aerobic exercise at various levels in honey-induced male mice. Methods. This study used an in vivo laboratory experimental method using a randomized complete block design (CRD). The study sample amounted to 30 mice, which were divided into six groups. Group (K-) is the group that was not given anything. The positive control group (K+1) was given 0.5 ml honey and open aerobic exercise but no oxygen. The positive control group (K+2) was given 0.5 ml honey and closed aerobic exercise, but no oxygen, and the treatment group (P1) was given 0.3 ml oxygen, 0.5 ml honey, and closed aerobic exercise. The treatment group (P2) was given 0.4 ml oxygen, 0.5 ml honey, and closed aerobic exercise. The treatment group (P3) was given 0.5 ml oxygen, 0.5 ml honey, and closed aerobic exercise. The maximum physical activity treatment was swimming and providing honey and oxygen in a closed aquarium for 7 days, which had previously been acclimatized for 7 days. Data were analyzed by one-way ANOVA at 0.05 level. Results. The results showed that oxygen administration had an effect (p < 0.05) on reducing the number of leukocyte levels. The most effective oxygen administration is in P3, with an oxygen concentration of 0.5 ml and 0.5 ml honey. Conclusion. Based on the results of the study, it can be concluded that aerobic exercise in the form of swimming in a closed aquarium that is given oxygen significantly reduces male mice leukocyte levels. © 2025, Society of Aging and Physical Activity (SAPA). All rights reserved.
Universitas Negeri Padang, Padang, Indonesia; Bandırma Onyedi Eylül Üniversitesi, Turkey; University of East Sarajevo, Bosnia and Herzegovina; University of Murcia, Spain
