Indra P. Jamaluddin, Susan H. Musa, Stalis N. Ethica, Arif N. M. Ansori, Valensa Yosephi, Peter Y. Atmaja, Ahmad A. A. Murtadlo, Sukma Sahadewa, Fara D. Durry, Maksim Rebezov, Marina Derkho, Sin W. Naw, Rahadian Zainul, Kadek Rachmawati
Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1). © 2024, Narra Sains Indonesia. All rights reserved.
Master of Immunology Study Program, Universitas Airlangga, Surabaya, Indonesia; Associate Degree Study Program of Health Analyst, Faculty of Nursing and Health Science, Universitas Muhammadiyah Semarang, Semarang, Indonesia; Master of Clinical Laboratory Science, Faculty of Nursing and Health Science, Universitas Muhammadiyah Semarang, Semarang, Indonesia; Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India; Virtual Research Center for Bioinformatics and Biotechnology, Surabaya, Indonesia; Soeradji Tirtonegoro Hospital, Klaten, Indonesia; Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; Faculty of Medicine, Universitas Wijaya Kusuma Surabaya, Surabaya, Indonesia; Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jawa Timur, Surabaya, Indonesia; Department of Scientific Research, V. M. Gorbatov Federal Research Center for Food Systems, Moscow, Russian Federation; Faculty of Biotechnology and Food Engineering, Ural State Agrarian University, Yekaterinburg, Russian Federation; Department of Natural Sciences, Institute of Veterinary Medicine, South Ural State Agrarian University, Troitsk, Russian Federation; Department of Chemistry, Myitkyina University, Myitkyina, Myanmar; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia; Center for Advanced Material Processing, Artificial Intelligence, and Biophysic Informatics (CAMPBIOTICS), Universitas Negeri Padang, Padang, Indonesia; Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
