Eko Susetyarini, Tutut Indria Permana, Supiana Dian Nurtjahyani, Rahadian Zainul, Vikash Jakhmola, Meksim Rebezov, Herland Satriawan
This study investigates the antifertility potential of Beluntas leaves (Pluchea indica) by its comparison with related species within the Pluchea genus through phylogenetic and bioinformatics approaches. By collecting genetic sequence data of Pluchea species from the GenBank NCBI database, analyses were conducted using MEGA X software to generate a phylogenetic tree, amino acid composition, and genetic distance table. Among the 13 Pluchea species analyzed, this research found that P. indica has significant potential as an antifertility agent based on its impact on the quality of male white rat spermatozoa, including motility and spermatogenic cell count. Furthermore, phylogenetic analysis indicates a close genetic relationship between P. indica and P. ovalis, suggesting the potential of P. ovalis as a new antifertility candidate. These results affirm the potential of Beluntas leaves as a male antifertility agent and open opportunities for further research on the potential of other Pluchea species. This study underscores the importance of bioinformatics studies in identifying plants for antifertility drugs, especially within the Pluchea genus, and suggests the need for further laboratory analysis on species closely related to P. indica to verify their antifertility potential. © 2024 by SPC.
Department of Biological Education, Faculty of Teaching and Education, Universitas Muhammadiyah Malang, Tlogomas Street No. 246, Post code 034146431, East Java, Malang, Indonesia; Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang, Jl. Raya Tlogomas No.246, Babatan, Tegalgondo, Kec. Lowokwaru, East Java, Malang City, 65144, Indonesia; Biology Educational, Postgraduate Programme, Universitas PGRI Ronggolawe, East Java, Indonesia, Jl. Manunggal No 61, East Java, Tuban, 62319, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatra, Padang, Indonesia; Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India; Department of Scientific Research, V. M. Gorbatov Federal Research Center for Food Systems, 26 Talalikhin Str, Moscow, 109316, Russian Federation; Faculty of Biotechnology and Food Engineering, Ural State Agrarian University, 42 Karl Liebknecht str, Yekaterinburg, 620075, Russian Federation; Institute of Ocean and Earth Sciences, University of Malaya, Kuala Lumpur, 50603, Malaysia
