Moralita Chatri, Poppy A. Z. Hasibuan, Edy Meiyanto, Deddi P. Putra, Endah P. Septisetyani, Denny Satria, Syukur B. Waruwu
Cancer is still a health problem in the world. The number of sufferers and deaths from cancer continues to increase from year to year, especially breast cancer. Cancer treatment is often associated with high toxicity and low selectivity, and the emergence of resistance to chemotherapeutic agents is a serious problem. Therefore, the development of new anticancer agents is a priority. This research provides information regarding the potential of the Vernonia amygdaline Delile plant for the development of breast cancer. Several studies show that this plant has the potential to be used as an anticancer agent. The research was conducted by extracting and fractionating this plant's leaves and then assessing the cytotoxic effect on T47D cell viability using Microtetrazolium. Next, cell cycle inhibition, apoptosis, p53 and Akt protein expression were analyzed using flow cytometry techniques. The results showed that the dichloromethane fraction had the highest yield. The dichloromethane fraction had the best IC50 value, 76.72 ± 1.79 μg/mL. Able to inhibit the cell cycle in the G2/M phase, stimulate apoptosis, increase p53 protein expression and inhibit Akt protein expression in T47D cells. The dichloromethane fraction can potentially be developed in treating breast cancer development. © 2024 Chatri et al.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia; Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia; Laboratory of Macromolecular Engineering, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia; Research Center for Genetic Engineering, National Research and Innovation Agency, Indonesia; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia; Faculty of Pharmacy and Health Sciences, Universitas Sari Mutiara Indonesia, Medan, Indonesia
