Riga Riga, Mauline Adia Silvani, Wandi Oktria, Suryelita Suryelita, Sri Benti Etika, Bali Yana Fitri, Sonni Maurit Benu, Mariam Ulfah, Fitri Yuranda
Fungal Phyllosticta capitalensis is reported to produce secondary metabolites with important bioactivities, i.e., antibacterial and antioxidant. This is the first time that the fungal P. capitalensis has been isolated from Andrographis paniculata twigs. Secondary metabolites isolation from the extract of the fungus P. capitalensis was performed using several techniques of chromatography, i.e., vacuum liquid chromatography and column chromatography, to obtain two pure compounds (1 and 2). Both compounds were determined for their structures using analyzing data of spectroscopy of NMR and FT-IR. Based on the analysis, compounds 1 and 2 were ergosterol and ergosterol peroxide, respectively. Both steroids were evaluated for their bioactivities following agar dilution method for antibacterial activity. Compound 2 was more active against three bacterial strains than compound 1. Based on the structure-activity relationship, presence of peroxide in C-5 and C-8 increases the bioactivity of ergosterol derivatives. Interestingly, herein, ergosterol peroxide (2) is firstly reported f from fungal P. capitalensis. © RJPT All right reserved.
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Jl. Prof. Dr. Hamka, Padang, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institute Teknologi Bandung, Jl. Ganesha 10, Bandung, Indonesia; Department of Pharmacy, STIKES Muhammadiyah Cirebon, Cirebon, Indonesia; Department of Environmental Engineering, Faculty of Engineering, Universitas Andalas, Padang, Indonesia
