Rahadian Zainul, Viol Dhea Kharisma, Santika Lusia Utami, Nelson Chandra, Arif Nur Muhammad Ansori, Arli Aditya Parikesit, Vikash Jakhmola, Daimon Syukri, Edi Syafri, Asri Peni Wulandari, Oski Illiandri, Khoirun Nisyak, Bahrun, Asmi Citra Malina A. R. Tasakka
Nowadays, dengue virus (DENV) is still become a global problem, even though the virus infection issues have reached half of the population in some countries each year. DENV belongs to the enveloped virus with positive-sense single-stranded RNA (+ssRNA) genus Flavivirus and belongs to the Flaviviridae family. DENV has structural proteins which consist of the envelope protein (E), capsid (C), and membrane (M). There are four serotypes of this virus which are DENV-1, 2, 3, and 4. These four serotypes are transmitted to humans through Aedes sp. The development of this vaccine is still in progress and the challenge of this DENV vaccine candidate design is to overcome the heterotypic infection and the expansion of coverage protection to all virus serotypes. This research uses design simulation for vaccine candidates using B cell epitope in all DENV’s serotypes envelope to trigger the antibody formation through bioinformatics method that consists of protein modeling, immunogenicity, toxicity, and immune stimulation. DENV envelope protein was predicted to have polytope that can be recognized by B cells and act as an antigen, have low similarity with the composing sequence of cell surface receptors on the body, and non-toxic, and then can trigger the population increase of B cell and IgM antibody production with high avidity to neutralize four of the DENV serotypes. We recommend the B cell polytype which consists of A, C, E, and G peptides be examined by the wet-lab approach. © RJPT All right reserved.
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia; Center for Advanced Material Processing, Artificial Intelligence, and Biophysic Informatics(CAMP-BIOTICS), Universitas Negeri Padang, Padang, Indonesia; Computational Virology Research Unit, Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia; Bioinformatics Department, Indonesia International Institute of Life Sciences (i3L), Jakarta Timur, Indonesia; Postgraduate School, Universitas Airlangga, Surabaya, Indonesia; Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India; Department of Food and Agriculture Product Technology, Faculty of Agriculture Technology, Universitas Andalas, Padang, Indonesia; Department of Agricultural Technology, Politeknik Pertanian Negeri Payakumbuh, Payakumbuh, Indonesia; Department of Biology, Faculty of Mathematics and Science, University of Padjadjaran, Bandung, Indonesia; Center for Bioprospecting of Natural Fibre and Bioresources, University of Padjadjaran, Bandung, Indonesia; Department of Biomedicine, School of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia; Department of Pharmacy, Faculty of Public Health, Universitas Anwar Medika, Sidoarjo, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia; Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, Indonesia
