Arif Nm. Ansori, Yulanda Antonius, Raden Jk. Susilo, Suhailah Hayaza, Viol D. Kharisma, Arli A. Parikesit, Rahadian Zainul, Vikash Jakhmola, Taru Saklani, Maksim Rebezov, Md. Emdad Ullah, Nikolai Maksimiuk, Marina Derkho, Pavel Burkov
CRISPR-Cas9 has emerged as a revolutionary tool that enables precise and efficient modifications of the genetic material. This review provides a comprehensive overview of CRISPR-Cas9 technology and its applications in genome editing. We begin by describing the fundamental principles of CRISPR-Cas9 technology, explaining how the system utilizes a single guide RNA (sgRNA) to direct the Cas9 nuclease to specific DNA sequences in the genome, resulting in targeted double-stranded breaks. In this review, we provide in-depth explorations of CRISPR-Cas9 technology and its applications in agriculture, medicine, environmental sciences, fisheries, nanotechnology, bioinformatics, and biotechnology. We also highlight its potential, ongoing research, and the ethical considerations and controversies surrounding its use. This review might contribute to the understanding of CRISPR-Cas9 technology and its implications in various fields, paving the way for future developments and responsible applications of this transformative technology. © 2023, Narra Sains Indonesia. All rights reserved.
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India; European Virus Bioinformatics Center, Jena, Germany; Faculty of Biotechnology, Universitas Surabaya, Surabaya, Indonesia; Nanotechology Engineering Study Program, Faculty of Advanced Technology and Multidiscipline, Universitas Airlangga, Surabaya, Indonesia; Doctoral Program of Mathematics and Natural Sciences, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; Generasi Biologi Indonesia Foundation, Gresik, Indonesia; Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences (i3L), Jakarta, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia; Department of Scientific Research, V. M. Gorbatov Federal Research Center for Food Systems, Moscow, Russian Federation; Faculty of Biotechnology and Food Engineering, Ural State Agrarian University, Yekaterinburg, Russian Federation; Department of Chemistry, Mississippi State University, MS, United States; Institute of Medical Education, Yaroslav-the-Wise Novgorod State University, Velikiy Novgorod, Russian Federation; Institute of Veterinary Medicine, South Ural State Agrarian University, Troitsk, Russian Federation
