Rini Elisia, Maiyontoni, Tuti Lestari, Fajri Maulana, Roni Jarlis, Romi Andika
The purpose of this study was to evaluate the optimal concentrations of glycerol and beta-carotene in Tris– egg yolk extender to maintain the semen quality of swamp buffalo. Semen was collected from two swamp buffalo bulls using an artificial vagina with a minimum semen motility standard of >70%. The research consisted of two stages. The first stage was the addition of glycerol and beta-carotene in Tris–egg yolk extender to evaluate the semen quality of swamp buffalo post-equilibration. The experimental method in the first stage used a randomized block design with eight treatments, namely A (control), B (glycerol 2%), C (glycerol 4%), D (glycerol 6%), E (glycerol 8%), F (beta-carotene 0.1%), G (beta-carotene 0.2%) and H (beta-carotene 0.3%), with equilibration times as blocks (0, 3 and 5 hours). The observed parameters were viability, motility and abnormality of swamp buffalo semen post-equilibration. The second stage was the addition of glycerol and beta-carotene in Tris–egg yolk extender to evaluate the semen quality of swamp buffalo post-thawing. The experimental method in the second stage also used a randomized block design with the same eight treatments (A–H) and three replications. The observed parameters were viability, motility, abnormality, and progressive sperm motility of swamp buffalo semen post-thawing. The results showed that fresh swamp buffalo semen had a volume of 2.26±1.15 ml, cream color, medium consistency, pH of 6.54±0.08, characteristic odor, concentration of 1.182±386.91 ×10⁶/ml, good mass motility (++) and motility of 71±2.00%. The addition of glycerol and beta-carotene in Tris–egg yolk extender significantly affected (p < 0.05) viability, motility and abnormality both post-equilibration and post-thawing, as well as post-thaw progressive sperm motility. The study concluded that the addition of 8% glycerol in Tris–egg yolk extender for swamp buffalo semen resulted in the best outcomes, with 65.57% viability post-equilibration, 78.12% motility post-equilibration, 35.58% abnormality post-equilibration, 61.63% viability post-thawing, 64.43% motility post-thawing, 31.28% abnormality post-thawing and 31.28% post-thaw progressive sperm motility. © 2026, ResearchersLinks Ltd. All rights reserved.
Animal Husbandry Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia; Department of Natural Science Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia; Department of Agricultural Industrial Technology, Study Program of Animal Feed Technology, Politeknik Negeri Tanah Laut, Tanah Laut, 70815, Indonesia; Department of Agroindustry, Study Program of Agribusiness, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia; Department of Animal Feed and Technology, Faculty of Animal Science, Universitas Andalas, Padang, 25163, Indonesia; Animal Science Study Program, Faculty of Animal Science, Universitas Andalas, Padang, 25163, Indonesia
