Rahmadanti Shabrina Haramain, Ade Andriani, Faiza Afroza, Urip Perwitasari, Des Saputro, Iwan Saskiawan
The exploration of natural ingredients for skincare applications has attracted growing scientific interest. Fermentation-based bioactives have emerged as a promising material as skincare ingredients with cosmeceutical properties, such as anti-aging, anti-acne, anti-inflammation and whitening activity. This study investigated the potency of laccase from Volvariella volvacea to be developed as a bioactive ingredient, especially for whitening and antioxidant. The fungus was cultivated under submerged fermentation using various natural organic media. Mung bean media (MB) resulted the highest laccase (Lac) activity up to 3400 U/L. The optimized condition for Lac production was observed at 5 % (w/v) MB and 18 incubation days (>5500 U/L). Lac has been well-known as melanin remover. In vitro degradation of melanin revealed the effectivity of Lac V. volvacea to remove melanin within 24 h in the presence of gallic acid as Lac mediator. Scanning Electron Microscope (SEM) analysis confirmed surface disruption of melanin granules after laccase treatment. GC/MS analysis also revealed that the fungus was effective on converting MB media into valuable products during fermentation. MB hydrolysates also were detected have antioxidant activity. The result shows that Lac derived from V. volvacea exhibits significant melanin-degrading activity and antioxidant properties, suggesting its potential as a natural, eco-friendly ingredient for skincare formulations. © 2026 Elsevier Ltd
Department of Biomedical Science, Faculty of Faculty of medicine, Andalas University, Padang, Indonesia; Research Center for Applied Microbiology, National Research and Innovation Agency, Jl. Raya Bogor, Km. 46, Cibinong, Bogor, 16911, Indonesia; Department of Biology, Faculty of Mathematics and Sciences, Universitas Negeri Padang, Padang, Indonesia
