E. Julita, A. Ulianas, M.S. Ahmad, I.M. Isa, Yulkifli, T.L. Ling, Y. Yolanda, Nurlely, M. Rezayi
An electrochemical cholesterol sensor based on emulsion photo-polymerized methacrylic acid molecularly imprinted polymers (MIPs) microspheres modified screen-printed electrode (SPE) has been developed. Electrochemical quantitation of cholesterol concentration was performed by means Cyclic Voltammetry (CV) and Square Wave Voltammetry (SWV) technique by using redox couple of potassium ferricyanide [K3Fe(CN)6]. A decrement in the electrochemical CV and SWV signal indicated that the cholesterol molecules were bound to the microspheres’ cavities of the biomimetic MIPs sensor, and formed an electron transfer barrier to the redox species from electron transfer at the electrode surface. Under optimal conditions, the electrochemical MIPs microsensor could detect cholesterol concentration in the linear response range of 0.5 mg L-1 to 50.0 mg L-1 with a limit of detection (LOD) at 0.1 mg L-1. The MIPs microspheres-modified carbon SPE electrode providing good operational stability to cholesterol sensing of up to 11 days long. © 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.
Department of Chemistry, Faculty of Mathematics and Science, Universitas Negeri Padang, Sumatera Barat, 25131, Indonesia; Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Tanjong Malim, Perak, 35900, Malaysia; Department of Physics, Faculty of Mathematics and Science, Universitas Negeri Padang, Sumatera, Indonesia; Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia UKM, Bangi, Selangor, 43600, Malaysia; Department of Chemistry, University of Ulsan, Ulsan, 44610, South Korea; Department of Physic, Faculty of Mathematics and Science, Universitas Indonesia, Jakarta, Indonesia; Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
