Minda Azhar, Syamsi Khairani, Ahadul Putra, Ike R. Daniel, Yuni Ahda, Ihsanawati, Fernita Puspasari, Dessy Natalia
Inulin-degrading bacteria from the dahlia tuber rhizosphere are potential sources of biocatalyst that hydrolyzed inulin to produce fructose and fructooligosaccharide (FOS). The research was done to identify the UKG isolate that screened from the dahlia tuber rhizosphere and determine the characterization of its extracellular inulin-degrading enzyme. The enzyme was purified using ammonium sulfate. Enzyme activity was determined using DNS reagent. Inulin degrading enzyme from Klebsiella variicola UKG isolate worked optimally at pH 5 and 40ºC. The enzyme showed endo-type action. The estimated molecular mass of the inulin degrading enzyme was 43 kDa. The enzyme had KM 63,317 mg/mL, and Vmax 0,274 µmol/mL.min. The enzyme is suitable for the production of FOS prebiotic from inulin. © 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.
Laboratory of Biochemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Jl. Prof. Dr. Hamka, Air Tawar, Padang, 25131, Indonesia; BBPOM Banda Aceh, Jl. Tgk. Mohd. Daud Beureueh, Banda Aceh, 23126, Indonesia; Laboratory of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Jl. Prof. Dr. Hamka, Air Tawar, Padang, 25131, Indonesia; Division of Biochemistry Research, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung, 40132, Indonesia
