D. Handayani, D.H. Putri, M. Oktaviani, Rahwani
Many of recent study were conducted to find and investigate new antimicrobial substance to overcome the bacterial resistance problem. We can explore antimicrobial substance from medicine plant including Andalas (Morus macroura Miq.), but this plant is an endangered species. So, we can use endophytic fungi from this plant since they were known have the ability to produce similar antimicrobial compound. The aim of this study is to find some endophytic fungi from Andalas plant that have the ability to inhibit pathogen growth. Part (roots and leaves) of Andalas plant sample are from Andaleh village, Tanah Datar district, West Sumatera. Part of plant surface sterilization was using NaOCl 0.5%. Isolation of endophytic fungi from Andaleh plant was using PDA containing Chloramphenichol 500mg/l. Antimicrobial activity assay was conducted by agar dilution method. This study using 3 microbial agent i.e. Escherichia coli, Staphylococcus aureus and Candida albicans. We found 19 pure isolates of endophytic fungi from roots sample meanwhile 9 pure isolates from leaves. Most of those endophytic fungi (24 of 28 isolates) have the ability to inhibit E.coli and S. aureus growth, but none of them have the same ability to inhibit C. albicans. The highest average inhibition zone against E. coli was 47.1 mm and 51.7 mm against S. aureus. Meanwhile the lowest average inhibition zone against E.coli and S. aureus was 13.6 mm. Based on this study, we can conclude that endophytic fungi from Andalas plant are potential source to develop antimicrobial agent. © Published under licence by IOP Publishing Ltd.
Departement of Biology, Faculty of Mathematics and Science (FMIPA), Universitas Negeri, Padang, Indonesia
