D.H. Putri, M. Fiffendy
One type of Dengue vaccine candidate that has the potential to be developed is DNA vaccine based on the DENV envelope gene. Constraints in developing vaccines are low secretion of envelope proteins. One of the efforts made to increase the secretion of envelope protein is by deletion the stem / anchor area. The purpose of this analysis was to analysis the effect of cutting the stem / anchor region on character and structure of the DENV E protein. Analysis of composition, profile, location and structure of proteins was using http://www.expasy.org, TMHMM, and http://bioinf.cs.ucl.ac.uk/psipred. Deletion of the stem-anchor region was changes the general properties of the protein, as well as the protein topology. However, the mutations did not change the 3D structure of main protein domain. © 2019 IOP Publishing Ltd.
Department of Biology, Faculty of Mathematics and Science (FMIPA), UniversitasNegeri Padang, Indonesia
