M. Azhar, D. Natalia, S. Syukur, N. Andriani, J. Jamsari
Inulin hydrolysis thermophilic and thermotolerant bacteria are potential sources of inulin hydrolysis enzymes. Partial gene that encodes inulin hydrolysis enzymes had been isolated from Bacillus subtilis using polymerase chain reaction (PCR) method with the DPE.slFandDPE.eR degenerative primers. The partial gene was cloned into pGEM-T Easy vector with E. coli as host cells and analyzed using BLASTx, CrustalW2, and Phyre2 programs. Size of thepartial gene had been found539 bp that encoded 179aminoacid residues of protein fragment. The sequences of protein fragment was more similar to exolevanase than exoinulinase. The protein fragment had conserved motif FSGS, and specific hits GH32 β-fructosidase. It had three residues of active site and five residues of substrate binding. The active site on the protein fragment were D (1-WLNDP-5), D (125-FRDPK-129) and E (177-WEC-179). Substrate binding on the protein fragment were ND (1-WLNDP-5), Q (18-FYQY-21), FS (60-FSGS-63) RD (125-FRDPK-129) and E (177-WEC-179). © Published under licence by IOP Publishing Ltd.
Biochemistry Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Jl. Prof. Hamka, Air Tawar, Padang, 25131, Indonesia; Biochemistry Research Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha10, Bandung, 40132, Indonesia; Biochemistry Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, 25163, Indonesia; Biotechnology Laboratory, Faculty of Agriculture, Universitas Andalas, Padang, 25163, Indonesia
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