Isolation and molecular identification of freshwater microalgae in Maninjau Lake West Sumatra

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Zulkarnain Chaidir, Neri Fadjria, Armaini, Rahadian Zainul

2016 Der Pharmacia Lettre Vol. 8 Issue 20 Article Cited by 14

Abstract

Microalgae are photosynthetic prokaryotic and eukaryotic microorganisms. In this research, isolation of microalgae from Maninjau lake West Sumatra was identified by morphological and molecular identification by PCR using primers for 16S rDNA and 18S rDNA prokaryotic and eukaryotic microalgae. The results of this study were obtained 3 isolates of microalgae that can be isolated from Maninjau lake West Sumatra are Scenedesmus (code isolate AUMA-020) with a percent similarity of 95%, Uncultured cyanobacterium (code isolates AUMA-023) with a percent similarity of 80% and Limnothrix (code isolates AUMA-019) has a percent similarity of 98% based on data gene Bank blast results.

Affiliations

University of Andalas Padang, Indonesia; Akademi Farmasi Dwi Farma Bukittinggi, Indonesia; Universitas Negeri Padang, Indonesia