R. Sumarmin, E. Yuniarti, A. Araucana, N.K. Huda
The quality of frozen semen may decrease during the freezing and thawing due to ice crystals and free radicals. Efforts to improve the quality of frozen semen can be done by modifying the diluents and the addition of cryoprotectant compounds. The bean sprouts extract contains antioxidant compounds that can function as a cryoprotectant in the diluent. This study aims to determine the effect of addition and optimum concentration of bean sprouts extract tris-quail yolk in the which can be used to improve the quality of frozen semen of Holstein Friesian Bull. This research consisted of 6 treatments (P) and 4 replications with Completely Randomized Design (CRD). P1 is tris-quail yolk with addition of 0.1%, 0.2% P2, P3 0.3%, 0.4% P4, and P5 0.5% of bean sprouts extracts. As for the control treatment (P0) is Tris-yolk quail without the addition of bean sprouts extract. The results Showed that the addition of 0.3% of bean sprouts extract haves Similar microscopic quality of frozen semen covering: motility, live sperm, acrosome cap, plasma membrane and intact abnormalities with the mean as follows 53.25%, 76.75%, 90.75%, 60.25% and 5:25% as normal as control. It can conclude that the combination of bean sprouts extract and tris-quail yolk can improvement the quality of frozen semen of Holstein Friesian Bull. © Published under licence by IOP Publishing Ltd.
Laboratory of Zoology, Department of Biology, Universitas Negeri Padang, Jl. Prof. Dr. HAMKA Freshwater Padang, 25260, Indonesia; Laboratory of Embryology, Department of Biology, Andalas University, Campus Limau Manis, Padang, 25321, Indonesia
