Melindra Mulia, Riga Riga, Ridha Wardatillah, Bali Yana Fitri, Sonni Maurit Benu, Mariam Ulfah, Fitri Yuranda, Ahadul Putra
A previous study showed that Andrographis paniculata is rich in bioactive compounds. To continue the study on chemical constituents from A. paniculata, fungus colonizing with A. paniculata was studied. In relation to that, this study aimed to identify secondary metabolites from fungus associated with the roots of A. paniculata, both qualitatively and quantitatively, as well as to evaluate their antibacterial and antioxidant bioactivities. The first step of this study was to inoculate the endophytic fungus from the roots of A. paniculata using the dilution method using Potato Dextrose Agar (PDA) as the medium, obtaining a strain of fungus labeled as RAP. Fungal RAP was identified as Fusarium solani using molecular identification. F. solani was fermented on three different rice media (white rice, red rice, and black rice) for three weeks, followed by extraction using ethyl acetate to give three crude extracts. The second step was the evaluation of all crude extracts for their chemical constituents. The results showed that all of them contained terpenoids, steroids, and phenolic compounds. Three crude extracts were also tested for antibacterial and antioxidant activity. The results of biological activity evaluations demonstrated that F. solani on white rice media showed the highest values of inhibition zone (11-13 mm) and the highest IC50 (53.72 μg/mL). Isolation and characterization of the bioactive compounds from the fungal F. solani obtained from the roots of A. paniculata will be investigated in another study. Interestingly, metabolite screening and the bioactivity of F. solani associated with A. paniculata are firstly reported herein. © 2025 Author(s).
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonesia; Department of Pharmacy, STIKES Muhammadiyah Cirebon, Cirebon, Indonesia; Department of Environmental Engineering, Faculty of Engineering, Universitas Andalas, Padang, Indonesia
